Molecular Genotyping of Human Adenovirus Infections from Gastrointestinal Tract and Conjunctivitis Patients
DOI:
https://doi.org/10.51699/cajmns.v7i1.3068Keywords:
Adenovirus, HAdV-D8, HAdV-D29, HAdV-D56, HAdV-D15, Conjunctivitis, Gastroenteritis, IraqAbstract
Human adenovirus is a common human viral pathogen especially of eye infection and gastroenteritis. This study includes detection of most circulating human adenovirus types implicated in both of conjunctivitis and gastroenteritis in Iraqi patients. Conjunctival swabs and stool samples taken separately from both of Keratoconjunctivitis and diarrheal patients with adenovirus infection. Viral DNA were taken and primers constructed specifically and then used to multiply the targeted gene. 10 positive specimens sent for sequencing for more analysis. The results revealed the out of around 80 patients of 10 samples give positive results for hexon gene and then sent for partial sequencing of hexon gene. The results showed that the local human adenovirus conjunctivitis isolates (IQC-1, IQC-3, and IQC-5) revealed close linked to human adenovirus type 8. The locally adenovirus conjunctivitis isolates (IQC2 and IQC4) and the local gastroenteritis isolates (IQ11 and IQI2) revealed closed linked to human adenovirus type 56. The locally gastroenteritis isolates (IQ13, IQI4) revealed close linked to human adenovirus type 29. The locally gastroenteritis isolates (IQI5) revealed close linked to human adenovirus type 15 at total genetic changes (0.20-0.050%). The current study demonstrated the potential implication of human adenovirus type D8 and D56 in eye infections especially conjunctivitis and HAdV-D29, D56, D15 in gastroenteritis.
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