Antimicrobial Effect of Fusarium solani (Endophytic fungi) Extract Associated with Coriandrum sativum L. plant
DOI:
https://doi.org/10.51699/cajmns.v4i6.2033Keywords:
ITS1 gene, endophytic, Fusarium solani, Coriandrum sativum, AntimicrbialAbstract
In this work, Fusarium solani (an endophytic fungus) was isolated from Coriandrum sativum L. healthy leaves. Its molecular identification was based on the ITS1 gene. Effect of endophytic fungal extract on a few pathogenic fungi and bacteria found at Tikrit University. In the current study, Fusarium solani was isolated from C. sativum leaves and showed 99% identity with genes in NCBI. Some secondary metabolites were detected in the extracts of F. solani and C. sativum which included total alkaloids, saponins, terpenoids, flavonoids (rutin 100 mg), and phenolic compounds (gallic acid 100mg). F. solani showed high total phenolic compounds (179.87 mg/ml) and showed significant activity against 29 isolated fungi belonging to Candida albicans, C.tropicalus, C.parapsilosis, C.krusei, C.kefyr, C.glabrata, and C.dubliniensis. The study showed the highest inhibition diameter of fungal isolation of C.kefyr was 39 mm at a concentration of 40 mg. ml and the lowest inhibition diameter was recorded in two fungi C.glabrata and C.dubliniensis with a diameter of 10.7 mm and a concentration of 20mg. ml. also the result showed that F. solani extract given high activity against 14 isolates of bacteria, the highest inhibition diameter of bacteria isolation of E.faecalis was 39.4 mm at a concentration of 40mg. ml and the lowest inhibition diameter was recorded in K.pneumoniae with a diameter of 10 mm and a concentration of 20mg. ml.
References
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31. Yu H, Zhang L, Li L, Zheng C, Guo L, Li W, Sun P, Qin L. Recent developments and prospects of antimicrobial metabolites produced by endophytes. Microbiological research. 2010 Aug 20;165(6):437-49.
32. Zhang D, Yang Y, Castlebury LA, Cerniglia CE. A method for the large-scale isolation of high transformation efficiency fungal genomic DNA. FEMS microbiology letters. 1996 Dec 1;145(2):261-5.
33. Zhao J, Shan T, Mou Y, Zhou L. Plant-derived bioactive compounds produced by endophytic fungi. Mini reviews in medicinal chemistry. 2011 Feb 1;11(2):159-68.
34. Zhu H, Wang Y, Liu Y, Xia Y, Tang T. Analysis of flavonoids in Portulaca oleracea L. by UV–vis spectrophotometry with comparative study on different extraction technologies. Food Analytical Methods. 2010 Jun; 3:90-7.
2. Aminov R. History of antimicrobial drug discovery: Major classes and health impact. Biochemical pharmacology. 2017 Jun 1;133:4-19.
3. Bacon CW, Glenn AE, Yates IE. Fusarium verticillioides: managing the endophytic association with maize for reduced fumonisins accumulation. Toxin Reviews. 2008 Jan 1;27(3-4):411-446.
4. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis. 2016 Apr 1;6(2):71-9.
5. Bokhari NA, Perveen K. In vitro inhibition potential of Phoenix dactylifera L. extracts on the growth of pathogenic fungi. Journal of Medicinal Plants Research. 2012 Feb 16;6(6):1083-1088.
6. Domsch KH, Gams W, Anderson TH. Compendium of soil fungi. vol. 1980; 1:511-2.
7. Herrera J, Khidir HH, Eudy DM, Porras-Alfaro A, Natvig DO, Sinsabaugh RL. Shifting fungal endophyte communities colonize Bouteloua gracilis: effect of host tissue and geographical distribution. Mycologia. 2010 Sep 1;102(5):1012-26.
8. Hyde KD, Xu J, Rapior S, Jeewon R, Lumyong S, Niego AG, Abeywickrama PD, Aluthmuhandiram JV, Brahamanage RS, Brooks S, Chaiyasen A. The amazing potential of fungi: 50 ways we can exploit fungi industrially. Fungal Diversity. 2019 Jul 31; 97:1-36.
9. Ibrahim SR, Abdallah HM, Elkhayat ES, Al Musayeib NM, Asfour HZ, Zayed MF, Mohamed GA. Fusaripeptide A: new antifungal and anti-malarial cyclodepsipeptide from the endophytic fungus Fusarium sp. Journal of Asian Natural Products Research. 2018 Jan 2;20(1):75-85.
10. Kaul S, Gupta S, Ahmed M, Dhar MK. Endophytic fungi from medicinal plants: a treasure hunt for bioactive metabolites. Phytochemistry reviews. 2012 Dec; 11:487-505.
11. Khan N, Afroz F, Begum MN, Rony SR, Sharmin S, Moni F, Hasan CM, Shaha K, Sohrab MH. Endophytic Fusarium solani: A rich source of cytotoxic and antimicrobial napthaquinone and aza-anthraquinone derivatives. Toxicology reports. 2018 Jan 1;5:970-976.
12. Kirk PM, Cannon PF, David JC, Stalpers JA. Ainsworth and Bisby's Dictionary of the Fungi. CABI publishing; 2001.
13. Kornsakulkarn J, Dolsophon K, Boonyuen N, Boonruangprapa T, Rachtawee P, Prabpai S, Kongsaeree P, Thongpanchang C. Dihydronaphthalenones from endophytic fungus Fusarium sp. BCC14842. Tetrahedron. 2011 Sep 30;67(39):7540-7.
14. Kumar S, Tamura K, Nei M. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in bioinformatics. 2004 Jun 1;5(2):150-63.
15. Latiffah Z, Nurul Izzati H, Baharuddin S. Fusarium species isolated from peat soil of Pondok Tanjung and Sungai Beriah, Perak. Malaysian Journal of Microbiology. 2010;6(1):102-5.
16. Lin JY, Tang CY. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food chemistry. 2007 Jan 1;101(1):140-7.
17. Liu HG, Li T, Zhao YL, Zhang J, Wang YZ. Determination of some metabolites of Cordyceps sobolifera. African Journal of Microbiology Research. 2011 Dec 16;5(30):5518-5522.
18. Mobeen A, Moazzam SW. Jawarish Shahi: A special dosage form of herbal formulations for functional gastrointestinal disorders in Unani medicine-A comprehensive review. Journal of Ethnopharmacology. 2022 Jul 15; 293:115319.
19. Mousavi SS, Karami A. Application of Endophyte microbes for production of secondary metabolites. Application of Microbes in Environmental and Microbial Biotechnology. 2022:1-37.
20. Palmer, L. T. and Kommedahl, T. 1969. Root-infecting Fusarium species in relation to root worm infestations in corn. Phytopathology, 59: 1613-1617.
21. Porras-Alfaro A, Bayman P. Hidden fungi, emergent properties: endophytes and microbiomes. Annual review of phytopathology. 2011 Sep 8; 49:291-315.
22. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Fungal Barcoding Consortium, Fungal Barcoding Consortium Author List, Bolchacova E, Voigt K. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the national academy of sciences. 2012 Apr 17;109(16):6241-6.
23. Schulz B, Wanke U, Draeger S, Aust HJ. Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycological research. 1993 Dec 1;97(12):1447-50.
24. Toghueo RM. Bioprospecting endophytic fungi from Fusarium genus as sources of bioactive metabolites. Mycology. 2020 Jan 2;11(1):1-21.
25. Von Nussbaum,F.,Brands,M.,Hinzen,B.,Weigand,S.,and Häbich,D Antibacterial natural products in medicinal chemistry-exodus or revival ? Angew.Chem. . 2006;45, 5072–5129.
26. Vu T, Hauschild R, Sikora RA. Fusarium oxysporum endophytes induced systemic resistance against Radopholus similis on banana. Nematology. 2006 Jan 1;8(6):847-852.
27. Walsh JL, Laurence MH, Liew EC, Sangalang AE, Burgess LW, Summerell BA, Petrovic T. Fusarium: two endophytic novel species from tropical grasses of northern Australia. Fungal Diversity. 2010 Oct; 44:149-159.
28. Wang QX, Li SF, Zhao F, Dai HQ, Bao L, Ding R, Gao H, Zhang LX, Wen HA, Liu HW. Chemical constituents from endophytic fungus Fusarium oxysporum. Fitoterapia. 2011 Jul 1;82(5):777-781.
29. Wen J, Okyere SK, Wang S, Wang J, Xie L, Ran Y, Hu Y. Endophytic fungi: an effective alternative source of plant-derived bioactive compounds for pharmacological studies. Journal of Fungi. 2022 Feb 20;8(2):205.
30. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfland DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York.
31. Yu H, Zhang L, Li L, Zheng C, Guo L, Li W, Sun P, Qin L. Recent developments and prospects of antimicrobial metabolites produced by endophytes. Microbiological research. 2010 Aug 20;165(6):437-49.
32. Zhang D, Yang Y, Castlebury LA, Cerniglia CE. A method for the large-scale isolation of high transformation efficiency fungal genomic DNA. FEMS microbiology letters. 1996 Dec 1;145(2):261-5.
33. Zhao J, Shan T, Mou Y, Zhou L. Plant-derived bioactive compounds produced by endophytic fungi. Mini reviews in medicinal chemistry. 2011 Feb 1;11(2):159-68.
34. Zhu H, Wang Y, Liu Y, Xia Y, Tang T. Analysis of flavonoids in Portulaca oleracea L. by UV–vis spectrophotometry with comparative study on different extraction technologies. Food Analytical Methods. 2010 Jun; 3:90-7.
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Published
2023-11-12
How to Cite
Mezher, M. A. . . (2023). Antimicrobial Effect of Fusarium solani (Endophytic fungi) Extract Associated with Coriandrum sativum L. plant. Central Asian Journal of Medical and Natural Science, 4(6), 299–307. https://doi.org/10.51699/cajmns.v4i6.2033
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